Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Applied Biosystems

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 January 1996:
Vol. 271. no. 5246, pp. 195 - 197
DOI: 10.1126/science.271.5246.195
Prev | Table of Contents | Next

Reports

Functional Evidence for Indirect Recognition of G·U in tRNAAla by Alanyl-tRNA Synthetase

K. Gabriel,  Jay Schneider,  William H. McClain (1)

The structural features of the G·U wobble pair in Escherichia coli alanine transfer RNA (tRNAAla) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNAAla and mutant tRNAs with G·U substitutions. tRNAAla with G·U, C·A, or G·A gave similar amounts of charged tRNAAla and supported viability of E. coli lacking chromosomal tRNAAla genes. tRNAAla with G·C was inactive. Recognition of G·U by AlaRS thus requires more than the functional groups on G·U in a regular helix and may involve detection of a helical distortion.


Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
(1) To whom correspondence should be addressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The wobble hypothesis revisited: Uridine-5-oxyacetic acid is critical for reading of G-ending codons.
S. J. Nasvall, P. Chen, and G. R. Bjork (2007)
RNA 13, 2151-2164
   Abstract »    Full Text »    PDF »
Trypanosoma Seryl-tRNA Synthetase Is a Metazoan-like Enzyme with High Affinity for tRNASec.
R. Geslain, E. Aeby, T. Guitart, T. E. Jones, M. C. de Moura, F. Charriere, A. Schneider, and L. R. de Pouplana (2006)
J. Biol. Chem. 281, 38217-38225
   Abstract »    Full Text »    PDF »
The Crystal Structure at 1.5 A Resolution of an RNA Octamer Duplex Containing Tandem G{middle dot}U Basepairs.
S. B. Jang, L.-W. Hung, M. S. Jeong, E. L. Holbrook, X. Chen, D. H. Turner, and S. R. Holbrook (2006)
Biophys. J. 90, 4530-4537
   Abstract »    Full Text »    PDF »
Surprising contribution to aminoacylation and translation of non-Watson-Crick pairs in tRNA.
W. H. McClain (2006)
PNAS 103, 4570-4575
   Abstract »    Full Text »    PDF »
Identity Elements of Archaeal tRNA..
B. Mallick, J. Chakrabarti, S. Sahoo, Z. Ghosh, and S. Das (2005)
DNA Res 12, 235-246
   Abstract »    Full Text »    PDF »
The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAProcmo5UGG promotes reading of all four proline codons in vivo.
S. J. NASVALL, P. CHEN, and G. R. BJORK (2004)
RNA 10, 1662-1673
   Abstract »    Full Text »    PDF »
A tRNAAla mutation causing mitochondrial myopathy clinically resembling myotonic dystrophy.
R Horvath, H Lochmuller, C Scharfe, B H Do, P J Oefner, J Muller-Hocker, B G Schoser, D Pongratz, D P Auer, and M Jaksch (2003)
J. Med. Genet. 40, 752-757
   Full Text »    PDF »
A yeast knockout strain to discriminate between active and inactive tRNA molecules.
R. Geslain, F. Martin, A. Camasses, and G. Eriani (2003)
Nucleic Acids Res. 31, 4729-4737
   Abstract »    Full Text »    PDF »
Modulation of tRNAAla identity by inorganic pyrophosphatase.
A. D. Wolfson and O. C. Uhlenbeck (2002)
PNAS 99, 5965-5970
   Abstract »    Full Text »    PDF »
Correlation of deformability at a tRNA recognition site and aminoacylation specificity.
K.-Y. Chang, G. Varani, S. Bhattacharya, H. Choi, and W. H. McClain (1999)
PNAS 96, 11764-11769
   Abstract »    Full Text »    PDF »
Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement.
M. Ibba, H. C. Losey, Y. Kawarabayasi, H. Kikuchi, S. Bunjun, and D. Soll (1999)
PNAS 96, 418-423
   Abstract »    Full Text »    PDF »
Sequences Outside Recognition Sets Are Not Neutral for tRNA Aminoacylation. EVIDENCE FOR NONPERMISSIVE COMBINATIONS OF NUCLEOTIDES IN THE ACCEPTOR STEM OF YEAST tRNAPhe.
M. Frugier, M. Helm, B. Felden, R. Giege, and C. Florentz (1998)
J. Biol. Chem. 273, 11605-11610
   Abstract »    Full Text »    PDF »
Evolution of a Transfer RNA Gene Through a Point Mutation in the Anticodon.
M. E. Saks, J. R. Sampson, and J. Abelson (1998)
Science 279, 1665-1670
   Abstract »    Full Text »
Identification in a pseudoknot of a U·G motif essential for the regulation of the expression of ribosomal protein S15.
L. Benard, N. Mathy, M. Grunberg-Manago, B. Ehresmann, C. Ehresmann, and C. Portier (1998)
PNAS 95, 2564-2567
   Abstract »    Full Text »    PDF »
Amber suppression in Escherichia coli by unusual mitochondria-like transfer RNAs.
V. Bourdeau, S. V. Steinberg, G. Ferbeyre, R. Emond, N. Cermakian, and R. Cedergren (1998)
PNAS 95, 1375-1380
   Abstract »    Full Text »    PDF »
The importance of tRNA backbone-mediated interactions with synthetase for aminoacylation.
W. H. McClain, J. Schneider, S. Bhattacharya, and K. Gabriel (1998)
PNAS 95, 460-465
   Abstract »    Full Text »    PDF »
Specific atomic groups and RNA helix geometry in acceptor stem recognition by a tRNA synthetase.
P. J. Beuning, F. Yang, P. Schimmel, and K. Musier-Forsyth (1997)
PNAS 94, 10150-10154
   Abstract »    Full Text »    PDF »
A curved RNA helix incorporating an internal loop with G·A and A·A non-Watson-Crick base pairing.
K. J. Baeyens, H. L. De Bondt, A. Pardi, and S. R. Holbrook (1996)
PNAS 93, 12851-12855
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)