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Science 8 March 1996:
Vol. 271. no. 5254, pp. 1416 - 1420
DOI: 10.1126/science.271.5254.1416
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Reports

Rearrangement-Enhancing Element Upstream of the Mouse Immunoglobulin Kappa Chain J Cluster

Laurent Ferradini, * Hua Gu, dagger Annie De Smet, Klaus Rajewsky, Claude-Agnès Reynaud, Jean-Claude Weill

Transcriptional regulatory elements have been shown to be necessary but not sufficient for the developmental regulation of immunoglobulin gene rearrangement in mouse precursor B cells. In the chicken lambda  light chain locus, additional elements in the V-J intervening sequence are involved in negative and positive regulation of rearrangement. Here, mutation of the mouse homolog of a chicken element, located in the Vkappa -Jkappa intervening sequence upstream of the Jkappa cluster, was shown to significantly decrease rearrangement. This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription.

L. Ferradini, A. De Smet, C.-A. Reynaud, J.-C. Weill, INSERM U373, Institut Necker, Faculté de Médecine Necker-Enfants Malades, Université Paris V, 156 rue de Vaugirard, 75730 Paris Cedex 05, France.
H. Gu and K. Rajewsky, Institut für Genetik, Universität Köln, Weyertal 121, D-50931 Köln, Germany.
* To whom correspondence should be addressed.
dagger Present address: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, MD 20852, USA.


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