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Science 10 May 1996:
Vol. 272. no. 5263, pp. 881 - 884
DOI: 10.1126/science.272.5263.881
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Reports

A U1/U4/U5 snRNP Complex Induced by a 2'-O-Methyl-Oligoribonucleotide Complementary to U5 snRNA

Gil Ast and Alan M. Weiner *

Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2'-O-methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA) nucleotides 68 to 88 (BU5Ae) disrupts the initial U4/U5/U6 tri-snRNP complex, enhances the U2/U6 interaction, and induces a U1/U4/U5 snRNP complex. The U1/U4/U5 snRNP complex interacts specifically with an RNA oligonucleotide containing the 5' splice site sequence and may therefore represent a transitional stage in the displacement of U1 from the 5' splice site by U5 snRNP.

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
* To whom correspondence should be addressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Proximity of the U12 snRNA with both the 5' Splice Site and the Branch Point during Early Stages of Spliceosome Assembly.
M. J. Frilander and X. Meng (2005)
Mol. Cell. Biol. 25, 4813-4825
   Abstract »    Full Text »    PDF »
The U1 snRNP Base Pairs with the 5' Splice Site within a Penta-snRNP Complex.
H. Malca, N. Shomron, and G. Ast (2003)
Mol. Cell. Biol. 23, 3442-3455
   Abstract »    Full Text »    PDF »
Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing.
N. Shomron, H. Malca, I. Vig, and G. Ast (2002)
Nucleic Acids Res. 30, 4127-4137
   Abstract »    Full Text »    PDF »
Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.
G. Ast, T. Pavelitz, and A. M. Weiner (2001)
Nucleic Acids Res. 29, 1741-1749
   Abstract »    Full Text »    PDF »
Synthetic Lethality of Yeast slt Mutations with U2 Small Nuclear RNA Mutations Suggests Functional Interactions between U2 and U5 snRNPs That Are Important for Both Steps of Pre-mRNA Splicing.
D. Xu, D. J. Field, S.-J. Tang, A. Moris, B. P. Bobechko, and J. D. Friesen (1998)
Mol. Cell. Biol. 18, 2055-2066
   Abstract »    Full Text »
Dynamics of the U1 Small Nuclear Ribonucleoprotein during Yeast Spliceosome Assembly.
S. W. Ruby (1997)
J. Biol. Chem. 272, 17333-17341
   Abstract »    Full Text »    PDF »


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