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Science 10 May 1996:
Vol. 272. no. 5263, pp. 884 - 886
DOI: 10.1126/science.272.5263.884
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Reports

Genetically Engineered Resistance to Dengue-2 Virus Transmission in Mosquitoes

K. E. Olson, S. Higgs, P. J. Gaines, A. M. Powers, B. S. Davis, K. I. Kamrud, J. O. Carlson, C. D. Blair, B. J. Beaty

The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.

Arthropod-Borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA.


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G. Attarzadeh-Yazdi, R. Fragkoudis, Y. Chi, R. W. C. Siu, L. Ulper, G. Barry, J. Rodriguez-Andres, A. A. Nash, M. Bouloy, A. Merits, et al. (2009)
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GROWTH CHARACTERISTICS OF CHIMERIVAX-DEN VACCINE VIRUSES IN AEDES AEGYPTI AND AEDES ALBOPICTUS FROM THAILAND.
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Am J Trop Med Hyg 75, 986-993
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Nairovirus RNA Sequences Expressed by a Semliki Forest Virus Replicon Induce RNA Interference in Tick Cells.
S. Garcia, A. Billecocq, J.-M. Crance, U. Munderloh, D. Garin, and M. Bouloy (2005)
J. Virol. 79, 8942-8947
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Inhibition of Flavivirus Infections by Antisense Oligomers Specifically Suppressing Viral Translation and RNA Replication.
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STUDIES ON CULTURED AND UNCULTURED MICROBIOTA OF WILD CULEX QUINQUEFASCIATUS MOSQUITO MIDGUT BASED ON 16S RIBOSOMAL RNA GENE ANALYSIS.
V. J. PIDIYAR, K. JANGID, M. S. PATOLE, and Y. S. SHOUCHE (2004)
Am J Trop Med Hyg 70, 597-603
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Nucleic Acid-Based Immune System: the Antiviral Potential of Mammalian RNA Silencing.
L. Gitlin and R. Andino (2003)
J. Virol. 77, 7159-7165
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RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome.
Z. N. Adelman, I. Sanchez-Vargas, E. A. Travanty, J. O. Carlson, B. J. Beaty, C. D. Blair, and K. E. Olson (2002)
J. Virol. 76, 12925-12933
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Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes.
C. D. Blair, Z. N. Adelman, and K. E. Olson (2000)
Clin. Microbiol. Rev. 13, 651-661
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Genetic manipulation of vectors: A potential novel approach for control of vector-borne diseases.
B. J Beaty (2000)
PNAS 97, 10295-10297
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Pathogen-specific resistance to Rift Valley fever virus infection is induced in mosquito cells by expression of the recombinant nucleoprotein but not NSs non-structural protein sequences.
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Inhibition of luciferase expression in transgenic Aedes aegypti mosquitoes by Sindbis virus expression of antisense luciferase RNA.
B. W. Johnson, K. E. Olson, T. Allen-Miura, A. Rayms-Keller, J. O. Carlson, C. J. Coates, N. Jasinskiene, A. A. James, B. J. Beaty, and S. Higgs (1999)
PNAS 96, 13399-13403
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An important step forward in the genetic manipulation of mosquito vectors of human disease.
M. G. Kidwell and A. R. Wattam (1998)
PNAS 95, 3349-3350
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Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly.
N. Jasinskiene, C. J. Coates, M. Q. Benedict, A. J. Cornel, C. S. Rafferty, A. A. James, and F. H. Collins (1998)
PNAS 95, 3743-3747
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Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria.
R. V. Durvasula, A. Gumbs, A. Panackal, O. Kruglov, S. Aksoy, R. B. Merrifield, F. F. Richards, and C. B. Beard (1997)
PNAS 94, 3274-3278
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PROMISING APPROACH TO PREVENTING DENGUE VIRUS INFECTION.
(1996)
Journal Watch (General) 1996, 4
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