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Science 26 July 1996:
Vol. 273. no. 5274, pp. 494 - 497
DOI: 10.1126/science.273.5274.494

Reports

Multicolor Spectral Karyotyping of Human Chromosomes

E. Schröck, S. du Manoir, T. Veldman, B. Schoell, J. Wienberg, M. A. Ferguson-Smith, Y. Ning, D. H. Ledbetter, I. Bar-Am, D. Soenksen, Y. Garini, T. Ried *

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

E. Schröck, S. du Manoir, T. Veldman, B. Schoell, Y. Ning, D. H. Ledbetter, T. Ried, Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, 49 Convent Drive, Building 49, Room 4A28, Bethesda, MD 20892-4470, USA.
J. Wienberg and M. A. Ferguson-Smith, Department of Pathology, Cambridge University, Cambridge CB21QP, UK.
I. Bar-Am, Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel.
D. Soenksen, Applied Spectral Imaging, Incorporated, Carlsbad, CA 92009, USA.
Y. Garini, Applied Spectral Imaging, Limited, Migdal Haemek, 10551 Israel.
*   To whom correspondence should be addressed. E-mail: tried{at}nchgr.nih.gov



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Astrocyte-specific Expression of Activated p21-ras Results in Malignant Astrocytoma Formation in a Transgenic Mouse Model of Human Gliomas.
H. Ding, L. Roncari, P. Shannon, X. Wu, N. Lau, J. Karaskova, D. H. Gutmann, J. A. Squire, A. Nagy, and A. Guha (2001)
Cancer Res. 61, 3826-3836
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Unraveling human cancer in the mouse: recent refinements to modeling and analysis.
L. Resor, T. J. Bowen, and A. Wynshaw-Boris (2001)
Hum. Mol. Genet. 10, 669-675
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Molecular cytogenetic characterisation of a complex 46,XY,t(7;8;11;13) chromosome rearrangement in a patient with Moebius syndrome.
G BORCK, J WIRTH, T HARDT, H TÖNNIES, K BRØNDUM-NIELSEN, M BUGGE, N TOMMERUP, H-G NOTHWANG, H-H ROPERS, and T HAAF (2001)
J. Med. Genet. 38, 117-121
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Human Papillomavirus Type 16 E6 Inactivation of p53 in Normal Human Mammary Epithelial Cells Promotes Tamoxifen-mediated Apoptosis.
V. L. Seewaldt, K. Mrózek, E. C. Dietze, M. Parker, and L. E. Caldwell (2001)
Cancer Res. 61, 616-624
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Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells.
B. Elenbaas, L. Spirio, F. Koerner, M. D. Fleming, D. B. Zimonjic, J. L. Donaher, N. C. Popescu, W. C. Hahn, and R. A. Weinberg (2001)
Genes & Dev. 15, 50-65
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New Approaches to Lymphoma Diagnosis.
N. L. Harris, H. Stein, S. E. Coupland, M. Hummel, R. D. Favera, L. Pasqualucci, and W. C. Chan (2001)
Hematology 2001, 194-220
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Fusion of the NH2-Terminal Domain of the Basic Helix-Loop-Helix Protein TCF12 to TEC in Extraskeletal Myxoid Chondrosarcoma with Translocation t(9;15)(q22;q21).
H. Sjögren, B. Wedell, J. M. M. Kindblom, L.-G. Kindblom, and G. Stenman (2000)
Cancer Res. 60, 6832-6835
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CHARGE association in a child with de novo chromosomal aberration 46,X,der(X)t(X;2)(p22.1;q33) detected by spectral karyotyping.
D. LEV, O. NAKAR, I. BAR-AM, A. ZUDIK, N. WATEMBERG, S. FINKELSTIEN, N. KATZIN, and T. LERMAN-SAGIE (2000)
J. Med. Genet. 37, 47e-47
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Acquired, nonrandom chromosomal abnormalities associated with the development of acute promyelocytic leukemia in transgenic mice.
D. B. Zimonjic, J. L. Pollock, P. Westervelt, N. C. Popescu, and T. J. Ley (2000)
PNAS 97, 13306-13311
   Abstract »    Full Text »    PDF »
New Tools in Molecular Pathology.
P. Lichter (2000)
J. Mol. Diagn. 2, 171-173
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Chromosome painting reveals specific patterns of chromosome occurrence in mitomycin C- and diethylstilboestrol-induced micronuclei.
E. Fauth, H. Scherthan, and H. Zankl (2000)
Mutagenesis 15, 459-467
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Immortal Human Pancreatic Duct Epithelial Cell Lines with Near Normal Genotype and Phenotype.
H. Ouyang, L.-j. Mou, C. Luk, N. Liu, J. Karaskova, J. Squire, and M.-S. Tsao (2000)
Am. J. Pathol. 157, 1623-1631
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Inactivation of 14-3-3sigma Influences Telomere Behavior and Ionizing Radiation-Induced Chromosomal Instability.
S. Dhar, J. A. Squire, M. P. Hande, R. J. Wellinger, and T. K. Pandita (2000)
Mol. Cell. Biol. 20, 7764-7772
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Abnormal rearrangement within the alpha /delta T-cell receptor locus in lymphomas from Atm-deficient mice.
M. Liyanage, Z. Weaver, C. Barlow, A. Coleman, D. G. Pankratz, S. Anderson, A. Wynshaw-Boris, and T. Ried (2000)
Blood 96, 1940-1946
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Differentially Painting Human Chromosome Arms with Combined Binary Ratio-labeling Fluorescence In Situ Hybridization.
J. Wiegant, V. Bezrookove, C. Rosenberg, H. J. Tanke, A. K. Raap, H. Zhang, M. Bittner, J. M. Trent, and P. Meltzer (2000)
Genome Res. 10, 861-865
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Seven-color Fluorescence Imaging of Tissue Samples Based on Fourier Spectroscopy and Singular Value Decomposition.
H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai (2000)
J. Histochem. Cytochem. 48, 653-662
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Genome changes and gene expression in human solid tumors.
J. W. Gray and C. Collins (2000)
Carcinogenesis 21, 443-452
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Correlation between semen parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men.
W. Vegetti, E. Van Assche, A. Frias, G. Verheyen, M. M. Bianchi, M. Bonduelle, I. Liebaers, and A. Van Steirteghem (2000)
Hum. Reprod. 15, 351-365
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Quantifying single gene copy number by measuring fluorescent probe lengths on combed genomic DNA.
J. Herrick, X. Michalet, C. Conti, C. Schurra, and A. Bensimon (2000)
PNAS 97, 222-227
   Abstract »    Full Text »    PDF »



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